Journal: iScience
Article Title: RBM11 drives malignant progression of bladder cancer by regulating GNPDA1-PKM2 axis
doi: 10.1016/j.isci.2026.115402
Figure Lengend Snippet: RBM11 promotes BCa progression by activating the GNPDA1 (A) RNA-seq in control and RBM11-overexpressing cells, KEGG assay revealed that DEGs significant enrichment in glycolytic pathway and extracellular matrix organization. (B) Among the coregulated genes, GNPDA1 and SPP1 showed the most pronounced upregulation. (C and D) RT-qPCR revealed that GNPDA1 was more significantly regulated by RBM11 ( n = 3 technical replicates from three biological replicates for each group). Data are presented as mean ± SD, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (unpaired t test). (E) RT-qPCR and WB revealed that OE-RBM11 significantly increased GNPDA1 while decreasing GNPDA2 ( n = 3 technical replicates from 3 biological replicates for each group). Data are presented as mean ± SD, ∗∗ p < 0.01 (unpaired t test). (F and G) Seahorse assay revealed that RBM11 overexpression promoted glycolysis (F), while GNPDA1 inhibition reduced it (G). n = 3 technical replicates from three biological replicates for each group. Data are presented as mean ± SD, ∗∗∗ p < 0.001 (two-way ANOVA). (H and I) Transwell assay revealed that downregulation of GNPDA1 attenuated the invasion and migration of BCa cells. n = 3 technical replicates from three biological replicates for each group. Scale bars, 100 μm. Data are presented as mean ± SD, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (one-way ANOVA). (J and K) Wound healing assay revealed that downregulation of GNPDA1 attenuated the migration of BCa cells. n = 3 technical replicates from three biological replicates for each group. Scale bars, 100 μm. Data are presented as mean ± SD, ∗∗ p < 0.01, ∗∗∗ p < 0.001 (one-way ANOVA).
Article Snippet: • The RNA-seq data have been submitted to the National Center for Biotechnology Information with the accession number PRJNA1426904 with full open access ( https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1426904 ).
Techniques: RNA Sequencing, Control, Quantitative RT-PCR, Over Expression, Inhibition, Transwell Assay, Migration, Wound Healing Assay